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Showing: 81 - 100 of 3836 results

  3. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-90684

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  4. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  5. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  9. pAAV-EF1a-DIO-HTB

    Plasmid
    #44187
    Depositor
    Edward Callaway
    Insert
    Histone tagged GFP, T2A-TVA-E2A-B19G
    Use
    AAV; Adeno-associated vector
    Tags
    GFP
    Expression
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-Ku70-TC

    Plasmid
    #46955
    Purpose
    Plasmid containing the targeting construct to insert a GFP-FLAG tag at the N-terminus of the human XRCC6 gene coding for Ku70.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    XRCC6 targeting construct (XRCC6 Human)
    Use
    AAV; R
    Tags
    GFP-FLAG
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    46955-plasmid-map-sequence-id-66844

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  19. pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47445
    Purpose
    AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NN_C63)-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Availability
    Academic Institutions and Nonprofits only
    47445-plasmid-map-sequence-id-72977

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  20. pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47446
    Purpose
    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5HD_C63)-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Availability
    Academic Institutions and Nonprofits only
    47446-plasmid-map-sequence-id-69438

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Showing: 81 - 100 of 3836 results